| 质粒名称: | pVITRO-HPV6 L1L2 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 52589 |
| 存储实验室: | Richard Roden |
|
载体骨架基本信息 |
|
| 载体名称: | pVITRO1-neo-mcs |
| 载体出品公司: | InvivoGen |
| 空载体大小: | 6295 bp |
| 完整质粒大小: | 9184 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 卡那霉素50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | HPV6 L1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1506 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BglII（克隆期间销毁） |
| 3’克隆位点: | NheI（克隆期间销毁） |
| 5’测序引物 | AAACCACCGCTAATTCAAAGC |
| 3’测序引物 | GTGGTTTGTCCAAACTCATCAA |
|
文献引用方法 |
|
| 材料和方法部分: | pVITRO-HPV6 L1L2 was a gift from Richard Roden (Addgene plasmid # 52589 ; http://n2t.net/addgene:52589 ; RRID:Addgene_52589) |
| 参考文献部分: | Impact of inhibitors and L2 antibodies upon the infectivity of diverse alpha and beta human papillomavirus types. Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB. PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232. eCollection 2014. 10.1371/journal.pone.0097232 PubMed 24816794 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pVITRO-HPV6 L1L2 9184 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses HPV6 L1 and L2 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pVITRO-HPV6 L1L2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9184)
AUTHORS Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
TITLE Impact of inhibitors and L2 antibodies upon the infectivity of
diverse alpha and beta human papillomavirus types.
JOURNAL PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
eCollection 2014.
PUBMED 24816794
REFERENCE 2 (bases 1 to 9184)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9184
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(74..94)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
intron 583..1530
/label=mEF-1-alpha intron
/note="intron upstream of the start codon of mouse
EF-1-alpha"
polyA_signal 3113..3234
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3124..3143)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 3178..3197
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
rep_origin 3422..4010
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3911..3930
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
enhancer 4090..4393
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
regulatory 5832..5841
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
misc_feature 7227..7671
/label=FMDV IRES
/note="internal ribosome entry site (IRES) of the
foot-and-mouth disease virus"
promoter 7704..7750
/label=EM7 promoter
/note="synthetic bacterial promoter "
promoter 7742..7760
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 7770..8564
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(7824..7843)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 8434..8453
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 8611..9183
/label=EF-1-alpha poly(A) signal
/note="human EF-1-alpha polyadenylation signal"
ORIGIN
1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
1561 aaccggtgat atcaaagatc cgccaccatg tggaggccca gcgacagcac cgtgtacgtg
1621 ccccccccca accccgtgag caaggtggtg gccaccgacg cctacgtgac caggaccaac
1681 atcttctacc acgccagcag cagcaggctg ctggccgtgg gccaccccta cttcagcatc
1741 aagagggcca acaagaccgt ggtgcccaag gtgagcggct accagtacag ggtgttcaag
1801 gtggtgctgc ccgaccccaa caagttcgcc ctgcccgaca gcagcctgtt cgaccccacc
1861 acccagaggc tggtgtgggc ctgcaccggc ctggaggtgg gcaggggcca gcccctgggc
1921 gtgggcgtga gcggccaccc cttcctgaac aagtacgacg acgtggagaa cagcggcagc
1981 ggcggcaacc ccggccagga caacagggtg aacgtgggca tggactacaa gcagacccag
2041 ctgtgcatgg tgggctgcgc cccccccctg ggcgagcact ggggcaaggg caagcagtgc
2101 accaacaccc ccgtgcaggc cggcgactgc ccccccctgg agctgatcac cagcgtgatc
2161 caggacggcg acatggtgga caccggcttc ggcgccatga acttcgccga cctgcagacc
2221 aacaagagcg acgtgcccat cgacatctgc ggcaccacct gcaagtaccc cgactacctg
2281 cagatggccg ccgaccccta cggcgacagg ctgttcttct tcctgaggaa ggagcagatg
2341 ttcgccaggc acttcttcaa cagggccggc gaggtgggcg agcccgtgcc cgacaccctg
2401 atcatcaagg gcagcggcaa caggaccagc gtgggcagca gcatctacgt gaacaccccc
2461 agcggcagcc tggtgagcag cgaggcccag ctgttcaaca agccctactg gctgcagaag
2521 gcccagggcc acaacaacgg catctgctgg ggcaaccagc tgttcgtgac cgtggtggac
2581 accaccagga gcaccaacat gaccctgtgc gccagcgtga ccaccagcag cacctacacc
2641 aacagcgact acaaggagta catgaggcac gtggaggagt acgacctgca gttcatcttc
2701 cagctgtgca gcatcaccct gagcgccgag gtgatggcct acatccacac catgaacccc
2761 agcgtgctgg aggactggaa cttcggcctg agcccccccc ccaacggcac cctggaggac
2821 acctacaggt acgtgcagag ccaggccatc acctgccaga agcccacccc cgagaaggag
2881 aagcccgacc cctacaagaa cctgagcttc tgggaggtga acctgaagga gaagttcagc
2941 agcgagctgg accagtaccc cctgggcagg aagttcctgc tgcagagcgg ctacaggggc
3001 aggagcagca tcaggaccgg cgtgaagagg cccgccgtga gcaaggccag cgccgccccc
3061 aagaggaaga gggccaagac caagaggtaa tagtctagct ggccagacat gataagatac
3121 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
3181 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
3241 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
3301 aagtaaaacc tctacaaatg tggtatggaa atgttaatta actagccatg accaaaatcc
3361 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
3421 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
3481 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
3541 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
3601 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
3661 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
3721 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
3781 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
3841 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
3901 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
3961 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
4021 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttcttaatta
4081 acctgcaggc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
4141 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
4201 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
4261 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
4321 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
4381 cgctattacc atgatgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
4441 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttgactagt
4501 ggagccgaga gtaattcata caaaaggagg gatcgccttc gcaaggggag agcccaggga
4561 ccgtccctaa attctcacag acccaaatcc ctgtagccgc cccacgacag cgcgaggagc
4621 atgcgcccag ggctgagcgc gggtagatca gagcacacaa gctcacagtc cccggcggtg
4681 gggggagggg cgcgctgagc gggggccagg gagctggcgc ggggcaaact gggaaagtgg
4741 tgtcgtgtgc tggctccgcc ctcttcccga gggtggggga gaacggtata taagtgcggt
4801 agtcgccttg gacgttcttt ttcgcaacgg gtttgccgtc agaacgcagg tgagtggcgg
4861 gtgtggcttc cgcgggcccc ggagctggag ccctgctctg agcgggccgg gctgatatgc
4921 gagtgtcgtc cgcagggttt agctgtgagc attcccactt cgagtggcgg gcggtgcggg
4981 ggtgagagtg cgaggcctag cggcaacccc gtagcctcgc ctcgtgtccg gcttgaggcc
5041 tagcgtggtg tccgccgccg cgtgccactc cggccgcact atgcgttttt tgtccttgct
5101 gccctcgatt gccttccagc agcatgggct aacaaaggga gggtgtgggg ctcactctta
5161 aggagcccat gaagcttacg ttggatagga atggaagggc aggaggggcg actggggccc
5221 gcccgccttc ggagcacatg tccgacgcca cctggatggg gcgaggcctg tggctttccg
5281 aagcaatcgg gcgtgagttt agcctacctg ggccatgtgg ccctagcact gggcacggtc
5341 tggcctggcg gtgccgcgtt cccttgcctc ccaacaaggg tgaggccgtc ccgcccggca
5401 ccagttgctt gcgcggaaag atggccgctc ccggggccct gttgcaagga gctcaaaatg
5461 gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg aagagggcct
5521 tgcccctcgc cggccgctgc ttcctgtgac cccgtggtct atcggccgca tagtcacctc
5581 gggcttctct tgagcaccgc tcgtcgcggc ggggggaggg gatctaatgg cgttggagtt
5641 tgttcacatt tggtgggtgg agactagtca ggccagcctg gcgctggaag tcattcttgg
5701 aatttgcccc tttgagtttg gagcgaggct aattctcaag cctcttagcg gttcaaaggt
5761 attttctaaa cccgtttcca ggtgttgtga aagccaccgc taattcaaag caatccggag
5821 tatacggatc cgccaccatg gcccacagca gggccaggag gaggaagagg gccagcgcca
5881 cccagctgta ccagacctgc aagctgaccg gcacctgccc ccccgacgtg atccccaagg
5941 tggagcacaa caccatcgcc gaccagatcc tgaagtgggg cagcctgggc gtgttcttcg
6001 gcggcctggg catcggcacc ggcagcggca ccggcggcag gaccggctac gtgcccctgc
6061 agaccagcgc caagcccagc atcaccagcg gccccatggc caggcccccc gtggtggtgg
6121 agcccgtggc ccccagcgac cccagcatcg tgagcctgat cgaggagagc gccatcatca
6181 acgccggcgc ccccgagatc gtgccccccg cccacggcgg cttcaccatc accagcagcg
6241 agaccaccac ccccgccatc ctggacgtga gcgtcaccag ccacaccacc accagcatct
6301 tcaggaaccc cgtgttcacc gagcccagcg tgacccagcc ccagcccccc gtggaggcca
6361 acggccacat cctgatcagc gcccccaccg tgaccagcca ccccatcgag gagatccccc
6421 tggacacctt cgtggtgagc agcagcgaca gcggccccac cagcagcacc cccgtgcccg
6481 gcaccgcccc caggcccagg gtgggcctgt acagcagggc cctgcaccag gtgcaggtga
6541 ccgaccccgc cttcctgagc accccccaga ggctgatcac ctacgacaac cccgtgtacg
6601 agggcgagga cgtgagcgtg cagttcagcc acgacagcat ccacaacgcc cccgacgagg
6661 ccttcatgga catcatcagg ctgcacaggc ccgccatcgc cagcaggagg ggcctggtga
6721 ggtacagcag gatcggccag aggggcagca tgcacaccag gagcggcaag cacatcggcg
6781 ccaggattca ctacttctac gacatcagcc ccatcgccca agccgccgag gagatcgaga
6841 tgcaccccct ggtggccgcc caggacgaca ccttcgacat ctacgccgag agcttcgagc
6901 ccggcatcaa ccccacccag caccccgtga ccaacatcag cgacacctac ctgaccagca
6961 cccccaacac cgtgacccag ccctggggca acaccaccgt gcccctgagc ctgcccaacg
7021 accccttcct gcagagcggc cccgacatca ccttccccac cgcccccatg ggcaccccct
7081 tcagccccgt gacccccgcc ctgcccaccg gccccgtgtt catcaccggc agcggcttct
7141 acctgcaccc cgcctggtac ttcgccagga agaggaggaa gaggattccc ctgttcttca
7201 gcgacgtggc cgcctaatag tctaggagca ggtttcccca atgacacaaa acgtgcaact
7261 tgaaactccg cctggtcttt ccaggtctag aggggtaaca ctttgtactg cgtttggctc
7321 cacgctcgat ccactggcga gtgttagtaa cagcactgtt gcttcgtagc ggagcatgac
7381 ggccgtggga actcctcctt ggtaacaagg acccacgggg ccaaaagcca cgcccacacg
7441 ggcccgtcat gtgtgcaacc ccagcacggc gactttactg cgaaacccac tttaaagtga
7501 cattgaaact ggtacccaca cactggtgac aggctaagga tgcccttcag gtaccccgag
7561 gtaacacgcg acactcggga tctgagaagg ggactggggc ttctataaaa gcgctcggtt
7621 taaaaagctt ctatgcctga ataggtgacc ggaggtcggc acctttcctt tgcaattact
7681 gaccctatga atacactgac tgtttgacaa ttaatcatcg gcatagtata tcggcatagt
7741 ataatacgac tcactatagg agggccacca tgattgaaca agatggattg cacgcaggtt
7801 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
7861 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
7921 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
7981 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
8041 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
8101 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
8161 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
8221 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
8281 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgaca catggcgatg
8341 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
8401 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
8461 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
8521 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
8581 cgaaatgacc gaccaagcga attcgctagg attatcccta atacctgcca ccccactctt
8641 aatcagtggt ggaagaacgg tctcagaact gtttgtttca attggccatt taagtttagt
8701 agtaaaagac tggttaatga taacaatgca tcgtaaaacc ttcagaagga aaggagaatg
8761 ttttgtggac cactttggtt ttcttttttg cgtgtggcag ttttaagtta ttagttttta
8821 aaatcagtac tttttaatgg aaacaacttg accaaaaatt tgtcacagaa ttttgagacc
8881 cattaaaaaa gttaaatgag aaacctgtgt gttcctttgg tcaacaccga gacatttagg
8941 tgaaagacat ctaattctgg ttttacgaat ctggaaactt cttgaaaatg taattcttga
9001 gttaacactt ctgggtggag aatagggttg ttttcccccc acataattgg aaggggaagg
9061 aatatcattt aaagctatgg gagggttgct ttgattacaa cactggagag aaatgcagca
9121 tgttgctgat tgcctgtcac taaaacaggc caaaaactga gtccttgggt tgcatagaaa
9181 gctg
//
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